Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
16751-1-AP targets CENPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CENPN fusion protein Ag10219 相同性解析による交差性が予測される生物種 |
| Full Name | centromere protein N |
| Calculated molecular weight | 339 aa, 40 kDa |
| Observed molecular weight | 40 kDa, 25-30 kDa |
| GenBank accession number | BC008972 |
| Gene Symbol | CENPN |
| Gene ID (NCBI) | 55839 |
| RRID | AB_2878310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96H22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPN antibody 16751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cancer Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.
| ||
Cancer Rep (Hoboken) High expression of centromere protein N as novel biomarkers for gastric adenocarcinoma
| ||
Horm Metab Res Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling Pathway |