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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CENPO Polyclonal antibody

CENPO Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20611-1-AP

Synonyms

CENP O, CENPO, centromere protein O, ICEN36, MCM21R

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse brain using 20611-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of HEK-293 using 20611-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse ovary using 20611-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis using 20611-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse brain using 20611-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse kidney using 20611-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HL-60 cells, mouse kidney tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20611-1-AP targets CENPO in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPO fusion protein Ag14619 相同性解析による交差性が予測される生物種
Full Name centromere protein O
Calculated molecular weight 300 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC002870
Gene symbol CENPO
Gene ID (NCBI) 79172
RRIDAB_10696179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for CENPO antibody 20611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols