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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CEP110 Polyclonal antibody (25235-1-AP)

    WB analysis of Jurkat using 25235-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25235-1-AP (CEP110 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CEP110 Polyclonal antibody (25235-1-AP)

    IF Staining of MDCK using 25235-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 25235-1-AP (CEP110 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CEP110 Polyclonal antibody

CEP110 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25235-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

WB, IF/ICC, ELISA

Synonyms

110 kDa centrosomal protein, Centriolin, Centrosomal protein 1, centrosomal protein 110kDa, CEP1, CEP110, FAN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25235-1-AP targets CEP110 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP110 fusion protein Ag18907 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 110kDa
Calculated molecular weight 2325 aa, 269 kDa
GenBank accession numberBC137284
Gene Symbol CEP110
Gene ID (NCBI) 11064
RRIDAB_2879979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7A1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CEP110 antibody 25235-1-APDownload protocol
IF protocol for CEP110 antibody 25235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols