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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP162 Polyclonal antibody

CEP162 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30210-1-AP

Synonyms

C6orf84, CEP162, FLJ13551, KIAA1009, Protein QN1 homolog, QN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP162 Polyclonal antibody (30210-1-AP)

    WB analysis using 30210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30210-1-AP (CEP162 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using CEP162 Polyclonal antibody (30210-1-AP)

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CEP162 Polyclonal antibody (30210-1-AP)

    IHC staining of human liver using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CEP162 Polyclonal antibody (30210-1-AP)

    IHC staining of human placenta using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CEP162 Polyclonal antibody (30210-1-AP)

    IHC staining of human placenta using 30210-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30210-1-AP (CEP162 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30210-1-AP targets CEP162 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP162 fusion protein Ag32953 相同性解析による交差性が予測される生物種
Full Name KIAA1009
Calculated molecular weight162 kDa
Observed molecular weight180 kDa
GenBank accession numberNM_001286206.1
Gene symbol CEP162
Gene ID (NCBI) 22832
RRIDAB_2935531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base.

Protocols

Product Specific Protocols
WB protocol for CEP162 antibody 30210-1-APDownload protocol
IHC protocol for CEP162 antibody 30210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols