Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 6 publications below |
Product Information
27325-1-AP targets CEP170 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEP170 fusion protein Ag26311 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 170kDa |
| Calculated molecular weight | 1486 aa, 165 kDa |
| Observed molecular weight | 170-190 kDa |
| GenBank accession number | BC140794 |
| Gene Symbol | CEP170 |
| Gene ID (NCBI) | 9859 |
| RRID | AB_2880844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SW79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP170 antibody 27325-1-AP | Download protocol |
| IF protocol for CEP170 antibody 27325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
J Cell Biol DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair. | ||
Elife α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.
| ||
Reprod Biomed Online A novel CCDC39 mutation causes multiple morphological abnormalities of the flagella in a primary ciliary dyskinesia patient | ||