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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CEP295 Polyclonal antibody (30446-1-AP)

    IHC staining of human prostate cancer using 30446-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30446-1-AP (CEP295 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CEP295 Polyclonal antibody (30446-1-AP)

    IHC staining of human prostate cancer using 30446-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30446-1-AP (CEP295 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CEP295 Polyclonal antibody (30446-1-AP)

    IF Staining of U2OS using 30446-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CEP295 antibody (30446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CEP295 Polyclonal antibody

CEP295 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30446-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

KIAA1731

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30446-1-AP targets CEP295 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP295 fusion protein Ag32896 相同性解析による交差性が予測される生物種
Full Name KIAA1731
Calculated molecular weight295 kDa
GenBank accession numberNM_033395.1
Gene Symbol CEP295
Gene ID (NCBI) 85459
RRIDAB_3669722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0D2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CEP295 antibody 30446-1-APDownload protocol
IF protocol for CEP295 antibody 30446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols