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CEP55 Monoclonal antibody

CEP55 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11F7

Cat no : 67460-1-Ig

Synonyms

Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis of HeLa using 67460-1-Ig

    WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis using 67460-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis using 67460-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using CEP55 Monoclonal antibody (67460-1-Ig)

    IHC staining of mouse adrenal gland using 67460-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using CEP55 Monoclonal antibody (67460-1-Ig)

    IHC staining of mouse adrenal gland using 67460-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HeLa using 67460-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HeLa using 67460-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HepG2 using 67460-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse testis tissue
Positive IHC detected inmouse adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67460-1-Ig targets CEP55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP55 fusion protein Ag29995 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 55kDa
Calculated molecular weight 54 kDa
Observed molecular weight 47-55 kDa
GenBank accession numberBC008947
Gene symbol CEP55
Gene ID (NCBI) 55165
RRIDAB_2882690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

Protocols

Product Specific Protocols
WB protocol for CEP55 antibody 67460-1-IgDownload protocol
IHC protocol for CEP55 antibody 67460-1-IgDownload protocol
IF protocol for CEP55 antibody 67460-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols