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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP63 Monoclonal antibody, PBS Only

CEP63 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G12B2

Cat no : 66996-1-PBS

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP63 Monoclonal antibody (66996-1-Ig)

    WB analysis using 66996-1-Ig (same clone as 66996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP63 Monoclonal antibody (66996-1-Ig)

    IF Staining of hTERT-RPE1 using 66996-1-Ig (same clone as 66996-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66996-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66996-1-PBS targets CEP63 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP63 fusion protein Ag9227 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 63kDa
Calculated molecular weight 703 aa, 81 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC014050
Gene symbol CEP63
Gene ID (NCBI) 80254
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.