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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP70 Polyclonal antibody

CEP70 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16280-1-AP

Synonyms

p10-binding protein, BITE

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human placenta tissue using CEP70 Polyclonal antibody (16280-1-AP)

    WB analysis of human placenta using 16280-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CEP70 Polyclonal antibody (16280-1-AP)

    WB analysis of PC-3 using 16280-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CEP70 Polyclonal antibody (16280-1-AP)

    WB analysis of mouse testis using 16280-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using CEP70 Polyclonal antibody (16280-1-AP)

    IHC staining of human ovary using 16280-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CEP70 Polyclonal antibody (16280-1-AP)

    IHC staining of human ovary using 16280-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP70 Polyclonal antibody (16280-1-AP)

    IF Staining of HeLa using 16280-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP70 antibody (16280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse testis tissue, PC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16280-1-AP targets CEP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP70 fusion protein Ag9365 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 70kDa
Calculated molecular weight 597 aa, 70 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC016050
Gene symbol CEP70
Gene ID (NCBI) 80321
RRIDAB_2077083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for CEP70 antibody 16280-1-APDownload protocol
IHC protocol for CEP70 antibody 16280-1-APDownload protocol
IF protocol for CEP70 antibody 16280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Front Endocrinol (Lausanne)

Identification of biallelic variations of CEP70 in patients with male infertility

Authors - Tiechao Ruan
View Article
humanWB,IP

Cell Insight

LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability

Authors - Xiaqing Zhang
View Article