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KD/KO Validated

CEP72 Polyclonal antibody

Name: CEP72 Polyclonal antibody
Brand: Proteintech
SKU: 19928-1-AP

CEP72 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19928-1-AP

Synonyms

KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of HEK-293 using 19928-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 19928-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19928-1-AP
IP result of anti-CEP72 (IP:19928-1-AP, 4ug; Detection:19928-1-AP 1:1000) with HeLa cells lysate 520ug.
IF Staining of HeLa using 19928-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP72 antibody (19928-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 19928-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP72 antibody (19928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 19928-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, HEK-293 cells, rat testis tissue
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

19928-1-AP targets CEP72 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CEP72 fusion protein Ag13905 相同性解析による交差性が予測される生物種
Full Name	centrosomal protein 72kDa
Calculated molecular weight	646 aa, 72 kDa
Observed molecular weight	72 kDa
GenBank accession number	BC001750
Gene symbol	CEP72
Gene ID (NCBI)	55722
RRID	AB_10638607
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CEP72 antibody 19928-1-AP	Download protocol
IF protocol for CEP72 antibody 19928-1-AP	Download protocol
IP protocol for CEP72 antibody 19928-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IP, IF	Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication. Authors - Andrew Kodani KD Validated View Article
human	IF	EMBO Rep Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. Authors - Andrea Martello View Article
human	IF	J Cell Sci Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. Authors - Deniz Conkar View Article
human	WB	Sci Rep Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors. Authors - Deniz Conkar KD Validated View Article
human	IF	J Biol Chem Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131). Authors - Ryan A Denu View Article
human	WB,IF	BMC Biol CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72 Authors - Zhenguang Ying View Article

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企業情報

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP72 Polyclonal antibody

CEP72 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 19928-1-AP

WB analysis using 19928-1-AP

IP experiment of HeLa using 19928-1-AP

IF Staining of HeLa using 19928-1-AP

IF Staining of HeLa using 19928-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.

Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131).

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72