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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP89, CCDC123 Polyclonal antibody

CEP89, CCDC123 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30165-1-AP

Synonyms

CEP89, CCDC123, Coiled-coil domain-containing protein 123, Cep123, Centrosomal protein of 89 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CEP89, CCDC123 Polyclonal antibody (30165-1-AP)

    WB analysis of A549 using 30165-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 30165-1-AP (CCDC123 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP89, CCDC123 Polyclonal antibody (30165-1-AP)

    IF Staining of hTERT-RPE1 using 30165-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CCDC123 antibody (30165-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inA549 cells
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30165-1-AP targets CEP89, CCDC123 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP89, CCDC123 fusion protein Ag32789 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 123
Calculated molecular weight 783 aa, 90 kDa
Observed molecular weight 85-100 kDa
GenBank accession numberBC136328
Gene symbol CEP89
Gene ID (NCBI) 84902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.

Protocols

Product Specific Protocols
WB protocol for CEP89, CCDC123 antibody 30165-1-APDownload protocol
IF protocol for CEP89, CCDC123 antibody 30165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols