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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CER1 Polyclonal antibody (18879-1-AP)

    WB analysis using 18879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18879-1-AP (CER1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CER1 Polyclonal antibody (18879-1-AP)

    IF Staining of mouse kidney using 18879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CER1 antibody (18879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CER1 Polyclonal antibody

CER1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 18879-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

CER1, Cerberus, Cerberus-related protein, DAND4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, mouse kidney tissue, rat kidney tissue
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18879-1-AP targets CER1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CER1 fusion protein Ag12926 相同性解析による交差性が予測される生物種
Full Name cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
Calculated molecular weight 267 aa, 30 kDa
Observed molecular weight70 kDa
GenBank accession numberBC069405
Gene Symbol CER1
Gene ID (NCBI) 9350
RRIDAB_3085568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. CER1 can exist as homo- or heterodimers (PMID: 23717584, 9501024).

Protocols

Product Specific Protocols
WB protocol for CER1 antibody 18879-1-APDownload protocol
IF protocol for CER1 antibody 18879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols