CES1 Polyclonal antibody
CES1 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 29041-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, rat liver tissue, hepG2 cells, Caco-2 cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C2C12 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29041-1-AP targets CES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CES1 fusion protein Ag30534 相同性解析による交差性が予測される生物種 |
Full Name | carboxylesterase 1 (monocyte/macrophage serine esterase 1) |
Calculated molecular weight | 566 aa, 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC012418 |
Gene symbol | CES1 |
Gene ID (NCBI) | 1066 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for CES1 antibody 29041-1-AP | Download protocol |
IHC protocol for CES1 antibody 29041-1-AP | Download protocol |
IF protocol for CES1 antibody 29041-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |