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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using CES1 Monoclonal antibody (67079-1-Ig)

    WB analysis of U-937 using 67079-1-Ig (same clone as 67079-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CES1 Monoclonal antibody (67079-1-Ig)

    WB analysis of THP-1 using 67079-1-Ig (same clone as 67079-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CES1 Monoclonal antibody (67079-1-Ig)

    IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CES1 Monoclonal antibody (67079-1-Ig)

    IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Monoclonal antibody (67079-1-Ig)

    IHC staining of human liver cancer using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Monoclonal antibody (67079-1-Ig)

    IHC staining of human liver cancer using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CES1 Monoclonal antibody (67079-1-Ig)

    IF Staining of HepG2 using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67079-1-Ig (CES1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

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CES1 Monoclonal antibody, PBS Only

CES1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67079-1-PBS
Clone No.1C11C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67079-1-PBS targets CES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CES1 fusion protein Ag10647 相同性解析による交差性が予測される生物種
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated molecular weight 566 aa, 62 kDa
Observed molecular weight 60-63 kDa
GenBank accession numberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
RRIDAB_2882387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP23141
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).