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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CES3 Polyclonal antibody

CES3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14587-1-AP

Synonyms

UNQ869/PRO1887, ES31, EC:3.1.1.1, Carboxylesterase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CES3 Polyclonal antibody (14587-1-AP)

    WB analysis using 14587-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14587-1-AP (CES3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CES3 Polyclonal antibody (14587-1-AP)

    IHC staining of human stomach cancer using 14587-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14587-1-AP (CES3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CES3 Polyclonal antibody (14587-1-AP)

    IF Staining of HeLa using 14587-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14587-1-AP (CES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14587-1-AP targets CES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES3 fusion protein Ag6113 相同性解析による交差性が予測される生物種
Full Name carboxylesterase 3
Calculated molecular weight 62 kDa
Observed molecular weight 60-62 kDa
GenBank accession numberBC053670
Gene symbol CES3
Gene ID (NCBI) 23491
RRIDAB_2275992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is expressed in liver, colon and small intestine. This antibody recognizes both isoforms with MW 60-62 kDa and 24 kDa.

Protocols

Product Specific Protocols
WB protocol for CES3 antibody 14587-1-APDownload protocol
IHC protocol for CES3 antibody 14587-1-APDownload protocol
IF protocol for CES3 antibody 14587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteome Res

Profiling impaired hepatic endoplasmic reticulum glycosylation as a consequence of ethanol ingestion.

Authors - Galligan James J JJ
View Article
humanWB

Environ Toxicol Pharmacol

Formaldehyde alters triglyceride synthesis and very low-density lipoprotein secretion in a time-dependent manner.

Authors - Jianying Bai
View Article