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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Centrin 3 Polyclonal antibody

Centrin 3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15811-1-AP

Synonyms

CEN3, Centrin 3, CETN3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Centrin 3 Polyclonal antibody (15811-1-AP)

    WB analysis using 15811-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15811-1-AP (Centrin 3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Centrin 3 Polyclonal antibody (15811-1-AP)

    IHC staining of mouse kidney using 15811-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Centrin 3 Polyclonal antibody (15811-1-AP)

    IHC staining of mouse kidney using 15811-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNCI-H1299 cells, C2C12 cells, Neuro-2a cells, mouse ovary tissue, mouse testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15811-1-AP targets Centrin 3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Centrin 3 fusion protein Ag8547 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 3 (CDC31 homolog, yeast)
Calculated molecular weight 167 aa, 20 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC005383
Gene symbol Centrin 3
Gene ID (NCBI) 1070
RRIDAB_2082361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Centrin 3 antibody 15811-1-APDownload protocol
IHC protocol for Centrin 3 antibody 15811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

Zika virus alters centrosome organization to suppress the innate immune response.

Authors - Andrew Kodani
View Article