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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CETP Polyclonal antibody (30136-1-AP)

    WB analysis using 30136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30136-1-AP (CETP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CETP Polyclonal antibody

CETP Polyclonal Antibody for WB, ELISA
Cat No. 30136-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

CETP, HDLCQ10, Lipid transfer protein I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HT-29 cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30136-1-AP targets CETP in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CETP fusion protein Ag32722 相同性解析による交差性が予測される生物種
Full Name cholesteryl ester transfer protein, plasma
Calculated molecular weight 493 aa, 55 kDa
Observed molecular weight55 kDa,70 kDa
GenBank accession numberBC025739
Gene Symbol CETP
Gene ID (NCBI) 1071
RRIDAB_3086242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cholesteryl ester transfer protein (CETP) is a plasma glycoprotein which transfers cholesteryl esters, triglycerides, and phospholipids between lipoproteins. In plasma, CETP is mainly associated with HDL particles and catalyzes the transfer of cholesteryl esters from HDL to apolipoprotein (apo) B-containing plasma lipoproteins. Low plasma concentrations of high-density lipoprotein-cholesterol (HDL-C) have long been recognized as a major, independent cardiovascular disease (CVD) risk factor. It demonstrated that altered level of plasma CETP was associated with the increased risk of CVD (PMID: 17991755, PMID: 23477743). CETP can be detected a 70 kDa isoform by glycosylation (PMID: 3281933,PMID: 22056562). It also can be detected 100 kDa dimer (PMID: 7744792).

Protocols

Product Specific Protocols
WB protocol for CETP antibody 30136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols