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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse testis tissue using CFAP70 Polyclonal antibody (31344-1-AP)

    IP experiment of mouse testis using 31344-1-AP

    IP result of anti-CFAP70 (IP:31344-1-AP, 4ug; Detection:31344-1-AP 1:300) with mouse testis tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CFAP70 Polyclonal antibody (31344-1-AP)

    IHC staining of rat testis using 31344-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CFAP70 Polyclonal antibody (31344-1-AP)

    IHC staining of mouse testis using 31344-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CFAP70 Polyclonal antibody (31344-1-AP)

    IF Staining of mouse testis using 31344-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CFAP70 antibody (31344-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CFAP70 Polyclonal antibody

CFAP70 Polyclonal Antibody for IHC, IF-P, IP, ELISA
Cat No. 31344-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, IP, ELISA

Synonyms

TTC18, TPR repeat protein 18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inmouse testis tissue
Positive IHC detected inrat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31344-1-AP targets CFAP70 in IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFAP70 fusion protein Ag35209 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 18
Calculated molecular weight126 kDa
Observed molecular weight 126 kDa
GenBank accession numberBC032856
Gene Symbol TTC18
Gene ID (NCBI) 118491
RRIDAB_3669954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T0N1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).

Protocols

Product Specific Protocols
IHC protocol for CFAP70 antibody 31344-1-APDownload protocol
IF protocol for CFAP70 antibody 31344-1-APDownload protocol
IP protocol for CFAP70 antibody 31344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols