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Complement factor B Polyclonal antibody

Complement factor B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10170-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman plasma, human blood, rat plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10170-1-AP targets Complement factor B in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag0226 相同性解析による交差性が予測される生物種
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93-100 kDa, 60-69 kDa
GenBank accession numberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
RRIDAB_2082384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00751
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Protocols

Product Specific Protocols
WB protocol for Complement factor B antibody 10170-1-APDownload protocol
IHC protocol for Complement factor B antibody 10170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Commun

The classical pathway triggers pathogenic complement activation in membranous nephropathy

Authors - Larissa Seifert
humanWB

J Cell Physiol

IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.

Authors - Xinxuan Cheng
humanWB,IHC,IF

Cell Oncol (Dordr)

Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.

Authors - Reiri Shimazaki
humanWB

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
human,mouseWB

Nephrol Dial Transplant

Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.

Authors - Ming Wu
WB

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky
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