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- KD/KO Validated
Factor D/CFD Polyclonal antibody
Factor D/CFD Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26050-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, human placenta tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
26050-1-AP targets Factor D/CFD in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Factor D/CFD fusion protein Ag23473 相同性解析による交差性が予測される生物種 |
| Full Name | complement factor D (adipsin) |
| Calculated molecular weight | 253 aa, 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC057807 |
| Gene symbol | CFD |
| Gene ID (NCBI) | 1675 |
| RRID | AB_2880352 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Factor D/CFD antibody 26050-1-AP | Download protocol |
| IHC protocol for Factor D/CFD antibody 26050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunol Res The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy. | ||
Hypertens Res Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation
|
