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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Complement factor H Polyclonal antibody

Complement factor H Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 12748-1-AP

Synonyms

CFH, FHL1, FH, Factor H, ARMS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Complement factor H Polyclonal antibody (12748-1-AP)

    WB analysis using 12748-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human blood using Complement factor H Polyclonal antibody (12748-1-AP)

    WB analysis of human blood using 12748-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using Complement factor H Polyclonal antibody (12748-1-AP)

    IP experiment of human plasma using 12748-1-AP

    IP result of anti-Complement factor H (IP:12748-1-AP, 4ug; Detection:12748-1-AP 1:1000) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human liver cancer using 12748-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human liver cancer using 12748-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human normal colon using 12748-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Complement factor H Polyclonal antibody (12748-1-AP)

    IHC staining of human normal colon using 12748-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Complement factor H Polyclonal antibody (12748-1-AP)

    IF Staining of human liver cancer using 12748-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Complement factor H Polyclonal antibody (12748-1-AP)

    IF Staining of HepG2 using 12748-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inhuman plasma, human blood
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12748-1-AP targets Complement factor H in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Complement factor H fusion protein Ag3482 相同性解析による交差性が予測される生物種
Full Name complement factor H
Calculated molecular weight 1231 aa, 139 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC037285
Gene symbol Complement factor H
Gene ID (NCBI) 3075
RRIDAB_10642946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

Protocols

Product Specific Protocols
WB protocol for Complement factor H antibody 12748-1-APDownload protocol
IHC protocol for Complement factor H antibody 12748-1-APDownload protocol
IF protocol for Complement factor H antibody 12748-1-APDownload protocol
IP protocol for Complement factor H antibody 12748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunol Res

The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.

Authors - Muyao Ye
View Article