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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11848-1-AP

Synonyms

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of human liver using 11848-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of mouse liver using 11848-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of human liver using 11848-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of L02 using 11848-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of mouse skeletal muscle using 11848-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of mouse lung using 11848-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    WB analysis of mouse skeletal muscle using 11848-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human skeletal muscle using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human normal colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human normal colon using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human heart using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human heart using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human heart using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human heart using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IHC staining of human gliomas using 11848-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Cofilin 2 Polyclonal antibody (11848-1-AP)

    IF Staining of L02 using 11848-1-AP

    Immunofluorescent analysis of L02 cells using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin 2 fusion protein Ag2408 相同性解析による交差性が予測される生物種
Full Name cofilin 2 (muscle)
Calculated molecular weight 166 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC022876
Gene symbol Cofilin 2
Gene ID (NCBI) 1073
RRIDAB_2080914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IHC protocol for Cofilin 2 antibody 11848-1-APDownload protocol
IF protocol for Cofilin 2 antibody 11848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

Authors - Lara Ottaviani
View Article