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  • Featured Product
  • KD/KO Validated

CFTR Monoclonal antibody, PBS Only

CFTR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4A8

Cat no : 66928-1-PBS

Synonyms

Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CFTR Monoclonal antibody (66928-1-Ig)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CFTR Monoclonal antibody (66928-1-Ig)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CFTR Monoclonal antibody (66928-1-Ig)

    WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

    WB result of CFTR antibody (66928-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and CFTR knockout HEK-293T cells. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CFTR Monoclonal antibody (66928-1-Ig)

    IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using CFTR Monoclonal antibody (66928-1-Ig)

    IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66928-1-PBS targets CFTR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CFTR fusion protein Ag27810 相同性解析による交差性が予測される生物種
Full Name cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Calculated molecular weight 168 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_000492
Gene symbol CFTR
Gene ID (NCBI) 1080
RRIDAB_2882254
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.