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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CGGBP1 Monoclonal antibody

CGGBP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5G9

Cat no : 66524-1-Ig

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CGGBP1 Monoclonal antibody (66524-1-Ig)

    WB analysis using 66524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66524-1-Ig (CGGBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using CGGBP1 Monoclonal antibody (66524-1-Ig)

    WB analysis of NIH/3T3 using 66524-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66524-1-Ig (CGGBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66524-1-Ig targets CGGBP1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CGGBP1 fusion protein Ag1129 相同性解析による交差性が予測される生物種
Full Name CGG triplet repeat binding protein 1
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC005222
Gene symbol CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2881887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Protocols

Product Specific Protocols
WB protocol for CGGBP1 antibody 66524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols