Validation Data Gallery
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Tested Applications
| Positive WB detected in | Caco-2 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
27633-1-AP targets CHAF1B in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHAF1B fusion protein Ag26504 相同性解析による交差性が予測される生物種 |
| Full Name | chromatin assembly factor 1, subunit B (p60) |
| Calculated molecular weight | 559 aa, 61 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC021218 |
| Gene Symbol | CHAF1B |
| Gene ID (NCBI) | 8208 |
| RRID | AB_2880933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHAF1B antibody 27633-1-AP | Download protocol |
| IHC protocol for CHAF1B antibody 27633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.
| ||
Cancer Res Commun Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells |