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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHAT Monoclonal antibody, PBS Only

CHAT Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7A9

Cat no : 66816-1-PBS

Synonyms

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHAT Monoclonal antibody (66816-1-Ig)

    WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using CHAT Monoclonal antibody (66816-1-Ig)

    WB analysis of PC-12 using 66816-1-Ig (same clone as 66816-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CHAT Monoclonal antibody (66816-1-Ig)

    IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66816-1-PBS targets CHAT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CHAT fusion protein Ag19602 相同性解析による交差性が予測される生物種
Full Name choline acetyltransferase
Calculated molecular weight 748 aa, 83 kDa
Observed molecular weight 67-71 kDa
GenBank accession numberBC130617
Gene symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_2882159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.