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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHCHD1 Polyclonal antibody

CHCHD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 11728-1-AP

Synonyms

Nuclear protein C2360, MRP-S37, MRPS37, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, C10orf34

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using CHCHD1 Polyclonal antibody (11728-1-AP)

    WB analysis of A431 using 11728-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11728-1-AP (CHCHD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CHCHD1 Polyclonal antibody (11728-1-AP)

    IHC staining of human stomach cancer using 11728-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11728-1-AP (CHCHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CHCHD1 Polyclonal antibody (11728-1-AP)

    IF Staining of A431 using 11728-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 11728-1-AP (CHCHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using CHCHD1 Polyclonal antibody (11728-1-AP)

    FC experiment of A431 using 11728-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CHCHD1 Polyclonal antibody (11728-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11728-1-AP targets CHCHD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD1 fusion protein Ag2325 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 1
Calculated molecular weight 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC020852
Gene symbol CHCHD1
Gene ID (NCBI) 118487
RRIDAB_2079769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHCHD1 antibody 11728-1-APDownload protocol
IHC protocol for CHCHD1 antibody 11728-1-APDownload protocol
IF protocol for CHCHD1 antibody 11728-1-APDownload protocol
FC protocol for CHCHD1 antibody 11728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article