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  • Featured Product
  • KD/KO Validated

CHCHD2 Polyclonal antibody, PBS Only

CHCHD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19424-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD2 Polyclonal antibody (19424-1-AP)

    WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD2 Polyclonal antibody (19424-1-AP)

    WB analysis of HEK-293 using 19424-1-AP (same clone as 19424-1-PBS)

    WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CHCHD2 Polyclonal antibody (19424-1-AP)

    WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using CHCHD2 Polyclonal antibody (19424-1-AP)

    WB analysis of human adrenal gland using 19424-1-AP (same clone as 19424-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CHCHD2 Polyclonal antibody (19424-1-AP)

    IP experiment of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHCHD2 Polyclonal antibody (19424-1-AP)

    IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHCHD2 Polyclonal antibody (19424-1-AP)

    IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHCHD2 Polyclonal antibody (19424-1-AP)

    IHC staining of mouse brain using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHCHD2 Polyclonal antibody (19424-1-AP)

    IHC staining of mouse brain using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Polyclonal antibody (19424-1-AP)

    IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Polyclonal antibody (19424-1-AP)

    IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Polyclonal antibody (19424-1-AP)

    IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody 19424-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19424-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD2 fusion protein Ag13752 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Calculated molecular weight 151 aa, 16 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC003079
Gene symbol CHCHD2
Gene ID (NCBI) 51142
RRIDAB_10638907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).