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  • Featured Product
  • KD/KO Validated

CHCHD2 Recombinant antibody

CHCHD2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12

Cat no : 82702-1-RR

Synonyms

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD2 Recombinant antibody (82702-1-RR)

    WB analysis using 82702-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD2 Recombinant antibody (82702-1-RR)

    WB analysis of HEK-293 using 82702-1-RR

    WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Recombinant antibody (82702-1-RR)

    IF Staining of HepG2 using 82702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82702-1-RR

    Affinity and Kinetic Characterization of 82702-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82702-1-RR targets CHCHD2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHCHD2 fusion protein Ag13752 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Calculated molecular weight 151 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC003079
Gene symbol CHCHD2
Gene ID (NCBI) 51142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Protocols

Product Specific Protocols
WB protocol for CHCHD2 antibody 82702-1-RRDownload protocol
IF protocol for CHCHD2 antibody 82702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols