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  • Featured Product
  • KD/KO Validated

CHCHD3 Monoclonal antibody

CHCHD3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8H12

Cat no : 68259-1-Ig

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis using 68259-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis using 68259-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CHCHD3 Monoclonal antibody (68259-1-Ig)

    WB analysis of HeLa using 68259-1-Ig

    WB result of CHCHD3 antibody (68259-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of human lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of human lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of human lung cancer using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of human lung cancer using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of mouse cerebellum using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of mouse cerebellum using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of mouse lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of mouse lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat cerebellum using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat cerebellum using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat heart using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat heart using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IHC staining of rat lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CHCHD3 Monoclonal antibody (68259-1-Ig)

    IF Staining of MCF-7 using 68259-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CHCHD3 antibody (68259-1-Ig, Clone: 1C8H12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig heart tissue, A549 cells, HeLa cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman lung tissue, human lung cancer tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat heart tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68259-1-Ig targets CHCHD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityrat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHCHD3 fusion protein Ag22628 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC014839
Gene symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Protocols

Product Specific Protocols
WB protocol for CHCHD3 antibody 68259-1-IgDownload protocol
IHC protocol for CHCHD3 antibody 68259-1-IgDownload protocol
IF protocol for CHCHD3 antibody 68259-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Ann Transl Med

Electroacupuncture ameliorates surgery-induced spatial memory deficits by promoting mitophagy in rats

Authors - Jian Guo
View Article