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CHCHD4 Polyclonal antibody

Name: CHCHD4 Polyclonal antibody
Brand: Proteintech
SKU: 21090-1-AP
Rating: 4.33 (3 reviews)

CHCHD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (3)

Cat no : 21090-1-AP

Synonyms

MIA40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Daudi using 21090-1-AP
Daudi cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 21090-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 21090-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 21090-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21090-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 21090-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21090-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 21090-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 21090-1-AP
Ramos cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21090-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 21090-1-AP
IP result of anti-CHCHD4 (IP:21090-1-AP, 4ug; Detection:21090-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.
IHC staining of mouse testis using 21090-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 21090-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21090-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 21090-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A431 using 21090-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHCHD4 antibody (21090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 21090-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Daudi cells, human heart tissue, mouse lung tissue, mouse spleen tissue, L02 cells, mouse kidney tissue, Ramos cells, Raji cells, rat brain tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 24 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

21090-1-AP targets CHCHD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHCHD4 fusion protein Ag15339 相同性解析による交差性が予測される生物種
Full Name	coiled-coil-helix-coiled-coil-helix domain containing 4
Calculated molecular weight	142 aa, 16 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC033775
Gene symbol	CHCHD4
Gene ID (NCBI)	131474
RRID	AB_10734583
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

Protocols

Product Specific Protocols
WB protocol for CHCHD4 antibody 21090-1-AP	Download protocol
IHC protocol for CHCHD4 antibody 21090-1-AP	Download protocol
IF protocol for CHCHD4 antibody 21090-1-AP	Download protocol
IP protocol for CHCHD4 antibody 21090-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. Authors - Rashim Pal Singh View Article
rat	WB,IF	Exp Mol Med SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis Authors - Jianxin Mao View Article
human	WB,IF	Aging Cell Dependence of PINK1 accumulation on mitochondrial redox system. Authors - Feng Gao KD Validated View Article
human	WB	Redox Biol Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner. Authors - Junchul Shin View Article
human	WB	EMBO Mol Med A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. Authors - Carina Lehmer View Article
human	WB	Oncogene CHTM1, a novel metabolic marker deregulated in human malignancies. Authors - Mansi Babbar View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Veronica (Verified Customer) (10-17-2023)	First time I find an anti-CHCHD4 antibody that works fine. We are testing it in IF as well, but till now I am already really happy with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: Gradient gel 8-15% Ab’: 1:1000 in 5% milk O/N Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293T; HeLa; isolated mitochondria from HEK293T CHCHD4 Antibody Western Blot validation (1:1000 dilution) in HEK293T; HeLa; isolated mitochondria from HEK293T (Cat no:21090-1-AP)
FH Süleyman (Verified Customer) (01-24-2022)	CHCHD4 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 as CHCHD4 supposed to be in IMS but I also see unspecific bindings in cytosolic proteins. Therefore my rating is 3 as it might be binding unspecifically. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: HeLa FlipIn CHCHD4 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:21090-1-AP)
FH Lana (Verified Customer) (08-13-2019)	SDS-PAGE: 10 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293t CHCHD4 Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:21090-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHCHD4 Polyclonal antibody

CHCHD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Daudi using 21090-1-AP

WB analysis of Raji using 21090-1-AP

WB analysis of rat brain using 21090-1-AP

WB analysis of human heart using 21090-1-AP

WB analysis of mouse lung using 21090-1-AP

WB analysis of mouse spleen using 21090-1-AP

WB analysis of L02 using 21090-1-AP

WB analysis of mouse kidney using 21090-1-AP

WB analysis of Ramos using 21090-1-AP

WB analysis of mouse brain using 21090-1-AP

IP experiment of mouse brain using 21090-1-AP

IHC staining of mouse testis using 21090-1-AP

IHC staining of mouse testis using 21090-1-AP

IHC staining of human kidney using 21090-1-AP

IHC staining of human kidney using 21090-1-AP

IF Staining of A431 using 21090-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Dependence of PINK1 accumulation on mitochondrial redox system.

Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner.

A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS.

CHTM1, a novel metabolic marker deregulated in human malignancies.

Reviews