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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHCHD4 Monoclonal antibody

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E4C6

Cat no : 66718-1-Ig

Synonyms

CHCHD4, FLJ31709, MIA40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD4 Monoclonal antibody (66718-1-Ig)

    WB analysis using 66718-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    WB analysis of rat brain using 66718-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IHC staining of human testis using 66718-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IHC staining of human testis using 66718-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SK-BR-3 cells using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IF Staining of SK-BR-3 using 66718-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inRaji cells, rat brain tissue, Ramos cells, Daudi cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66718-1-Ig targets CHCHD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHCHD4 fusion protein Ag15339 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033775
Gene symbol CHCHD4
Gene ID (NCBI) 131474
RRIDAB_2882069
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

Protocols

Product Specific Protocols
WB protocol for CHCHD4 antibody 66718-1-IgDownload protocol
IHC protocol for CHCHD4 antibody 66718-1-IgDownload protocol
IF protocol for CHCHD4 antibody 66718-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols