Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IF | See 1 publications below |
Product Information
20639-1-AP targets CHCHD6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHCHD6 fusion protein Ag14700 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil-helix-coiled-coil-helix domain containing 6 |
| Calculated molecular weight | 235 aa, 26 kDa |
| Observed molecular weight | 26-29 kDa |
| GenBank accession number | BC006123 |
| Gene Symbol | CHCHD6 |
| Gene ID (NCBI) | 84303 |
| RRID | AB_10697667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| IHC protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| IF protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| IP protocol for CHCHD6 antibody 20639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. | ||
EMBO Rep Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner. | ||
Elife QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease. | ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
| ||
Sci Rep Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
| ||
Biochim Biophys Acta Biomembr Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes. |