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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHCHD6 Monoclonal antibody, PBS Only (Capture)

CHCHD6 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11E9

Cat no : 66597-1-PBS

Synonyms

MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CHCHD6 Monoclonal antibody (66597-1-Ig)

    WB analysis using 66597-1-Ig (same clone as 66597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66597-1-Ig (CHCHD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66597-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CHCHD6 Monoclonal antibody (66597-1-Ig)

    IF Staining of MCF-7 using 66597-1-Ig (same clone as 66597-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66597-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50477-2

    Cytometric bead array standard curve of MP50477-2

    Cytometric bead array standard curve of MP50477-2, CHCHD6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66597-1-PBS. Detection antibody: 66597-4-PBS. Standard:Ag14700. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP50477-4

    Cytometric bead array standard curve of MP50477-4

    Cytometric bead array standard curve of MP50477-4, CHCHD6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66597-1-PBS. Detection antibody: 66597-3-PBS. Standard:Ag14700. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66597-1-PBS targets CHCHD6 as part of a matched antibody pair:

MP50477-2: 66597-1-PBS capture and 66597-4-PBS detection (validated in Cytometric bead array)

MP50477-4: 66597-1-PBS capture and 66597-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHCHD6 fusion protein Ag14700 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 6
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 26-29 kDa
GenBank accession numberBC006123
Gene symbol CHCHD6
Gene ID (NCBI) 84303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.