Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
12311-1-AP targets CHD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHD2 fusion protein Ag2960 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain helicase DNA binding protein 2 |
| Calculated molecular weight | 1828 aa, 211 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC020810 |
| Gene Symbol | CHD2 |
| Gene ID (NCBI) | 1106 |
| RRID | AB_2079925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14647 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chromodomain-helicase-DNA-binding protein 2, also named as ATP-dependent helicase CHD2, CHD2. It is a sequence-selective DNA-binding protein. Molecular mass of CHD2 is 211kD (201kd for isoform 2, 57kd for isoform 3). CHD2 is confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD2 antibody 12311-1-AP | Download protocol |
| IHC protocol for CHD2 antibody 12311-1-AP | Download protocol |
| IP protocol for CHD2 antibody 12311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
PeerJ Identification and validation of immune related core transcription factors GTF2I in NAFLD. | ||