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- KD/KO Validated
CHD4 Polyclonal antibody
CHD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 14173-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
14173-1-AP targets CHD4 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHD4 fusion protein Ag5372 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain helicase DNA binding protein 4 |
| Calculated molecular weight | 1937 aa, 221 kDa |
| Observed molecular weight | 260 kDa |
| GenBank accession number | BC038596 |
| Gene symbol | CHD4 |
| Gene ID (NCBI) | 1108 |
| RRID | AB_2080046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IHC protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IF protocol for CHD4 antibody 14173-1-AP | Download protocol |
| IP protocol for CHD4 antibody 14173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. | ||
Leukemia Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development. | ||
Cancer Res CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.
| ||
Arthritis Rheumatol Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis. | ||
Clin Epigenetics CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.
| ||
Cell Signal CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xiaochen (Verified Customer) (07-31-2023) | It is very sensitive and accurate. I can get the sepecific bend easily.
 |
FH Christos (Verified Customer) (01-26-2023) | Application: IF Sample: Human Cell line (HeLa Kyoto) Fixation: Paraformaldehyde (PFA 4% in PHEM pH=6.9) at room temperature for 15 minutes Permeabilization: 0.1% Triton-X-100 in PBS at room temperature for 5 minutes Blocking step: BSA as blocking agent for 1 hour · Concentration: 5% BSA in PBS · Temperature: RT dilution of the primary antibody: 1/50 incubation time: overnight at 4°C DNA (blue), CHD4 (green) and α-tubulin (red)
 |
