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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    WB result of CHD4 antibody (66222-1-Ig, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of MCF-7 using 66222-1-Ig (same clone as 66222-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHD4 Monoclonal antibody (66222-1-Ig)

    IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHD4 Monoclonal antibody (66222-1-Ig)

    IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    IF Staining of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HEK-293 cells using 66222-1-Ig(CHD4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CHD4 Monoclonal antibody, PBS Only

CHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66222-1-PBS
Clone No.3D9G2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66222-1-PBS targets CHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHD4 fusion protein Ag5729 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 4
Calculated molecular weight 1937 aa, 221 kDa
Observed molecular weight 260 kDa
GenBank accession numberBC038596
Gene Symbol CHD4
Gene ID (NCBI) 1108
RRIDAB_2881613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14839
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.