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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHD5 Polyclonal antibody (22725-1-AP)

    WB analysis using 22725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22725-1-AP (CHD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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CHD5 Polyclonal antibody

CHD5 Polyclonal Antibody for WB, ELISA
Cat No. 22725-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ATP dependent helicase CHD5, ATP-dependent helicase CHD5, CHD 5, CHD-5, Chromodomain-helicase-DNA-binding protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22725-1-AP targets CHD5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHD5 fusion protein Ag18621 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 5
Calculated molecular weight 1954 aa, 223 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC148803
Gene Symbol CHD5
Gene ID (NCBI) 26038
RRIDAB_3669403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TDI0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHD5 antibody 22725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols