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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHD7 Polyclonal antibody (31919-1-AP)

    WB analysis using 31919-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 31919-1-AP (CHD7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CHD7 Polyclonal antibody (31919-1-AP)

    IF Staining of HEK-293 using 31919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CHD7 antibody (31919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CHD7 Polyclonal antibody

CHD7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31919-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1416, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 7, Chromodomain Helicase DNA Binding Protein 7, CHD-7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HuH-7 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31919-1-AP targets CHD7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHD7 fusion protein Ag36595 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 7
Observed molecular weight350 kDa
GenBank accession numberBC110818
Gene Symbol CHD7
Gene ID (NCBI) 55636
RRIDAB_3670144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P2D1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent eukaryotic chromatin remodeling enzyme that regulates nucleosome positioning and alters DNA accessibility, and is essential for organ development.CHD7 is a gene known to be associated with CHARGE syndrome, Kallmann syndrome, and hypogonadotropic hypogonadism, where it is associated with CHARGE syndrome is a congenital multiorgan disorder characterized by eye defects, heart defects, posterior nasal atresia, growth retardation, genital anomalies, ear malformations, and deafness. The effects of CHD7 mutations on inner ear development, neuronal differentiation, cardiovascular development, and regulation of bone lipid homeostasis have been studied.

Protocols

Product Specific Protocols
WB protocol for CHD7 antibody 31919-1-APDownload protocol
IF protocol for CHD7 antibody 31919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols