Validation Data Gallery
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Tested Applications
| Positive WB detected in | SGC-7901 cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
13402-1-AP targets CHD9 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHD9 fusion protein Ag4245 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain helicase DNA binding protein 9 |
| Calculated molecular weight | 2897 aa, 326 kDa |
| Observed molecular weight | 330 kDa |
| GenBank accession number | BC033770 |
| Gene Symbol | CHD9 |
| Gene ID (NCBI) | 80205 |
| RRID | AB_10640425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3L8U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IHC protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IF protocol for CHD9 antibody 13402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Carcinog miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.
| ||
Braz J Med Biol Res Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer. | ||
Int J Clin Exp Pathol High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma. | ||
mBio A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.
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