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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHERP Polyclonal antibody

CHERP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25389-1-AP

Synonyms

CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CHERP Polyclonal antibody (25389-1-AP)

    WB analysis of Jurkat using 25389-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CHERP Polyclonal antibody (25389-1-AP)

    WB analysis using 25389-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of mouse brain using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of mouse testis using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of human kidney using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHERP Polyclonal antibody (25389-1-AP)

    IHC staining of mouse brain using 25389-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inJurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25389-1-AP targets CHERP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHERP fusion protein Ag13428 相同性解析による交差性が予測される生物種
Full Name calcium homeostasis endoplasmic reticulum protein
Calculated molecular weight 104 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC021294
Gene symbol CHERP
Gene ID (NCBI) 10523
RRIDAB_2880055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHERP antibody 25389-1-APDownload protocol
IHC protocol for CHERP antibody 25389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols