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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23342-1-AP

Synonyms

CHGA, CGA, ER-37, EA-92, Chromogranin-A

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human small intestine using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human small intestine using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human appendicitis using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse pancreas using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human colon using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human colon using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse colon using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of mouse colon using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IHC staining of human lung cancer using 23342-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IF Staining of mouse small intestine using 23342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Chromogranin A Polyclonal antibody (23342-1-AP)

    IF Staining of mouse small intestine using 23342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Chromogranin A Polyclonal antibody (23342-1-AP)

    IF Staining of Neuro-2a using 23342-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Chromogranin A antibody (23342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse pancreas tissue, human lung cancer tissue, human small intestine tissue, human colon tissue, mouse colon tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23342-1-AP targets Chromogranin A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin A fusion protein Ag19780 相同性解析による交差性が予測される生物種
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
GenBank accession numberBC006459
Gene symbol Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2879259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocols

Product Specific Protocols
IHC protocol for Chromogranin A antibody 23342-1-APDownload protocol
IF protocol for Chromogranin A antibody 23342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.

Authors - Buqing Ye
View Article
mouseIF

Cell Mol Gastroenterol Hepatol

Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia

Authors - Pawan Puri
View Article