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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30419-1-AP

Synonyms

CHI3L1, GP 39, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHI3L1 Polyclonal antibody (30419-1-AP)

    WB analysis using 30419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30419-1-AP (CHI3L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30419-1-AP targets CHI3L1/YKL40 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHI3L1/YKL40 fusion protein Ag33123 相同性解析による交差性が予測される生物種
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC008568
Gene symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Protocols

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 30419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols