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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHM Polyclonal antibody

CHM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26251-1-AP

Synonyms

Choroideraemia protein, REP1, REP 1, Rab escort protein 1, GGTA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHM Polyclonal antibody (26251-1-AP)

    WB analysis using 26251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26251-1-AP (CHM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using CHM Polyclonal antibody (26251-1-AP)

    IHC staining of mouse placenta using 26251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26251-1-AP (CHM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CHM Polyclonal antibody (26251-1-AP)

    IF Staining of A431 using 26251-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHM antibody (26251-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inA431 cells, Caco-2 cells, HEK-293T cells, HeLa cells
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26251-1-AP targets CHM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHM fusion protein Ag23166 相同性解析による交差性が予測される生物種
Full Name choroideremia (Rab escort protein 1)
Observed molecular weight 95 kDa
GenBank accession numberBC063522
Gene symbol CHM
Gene ID (NCBI) 1121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHM (Choroideremia protein) is also named as Rab escort protein 1 (REP-1), TCD and Rab proteins geranylgeranyltransferase component A 1. Choroideremia (CHM) is an X-linked recessive eye disease that is caused by null mutations in REP-1, a gene involved in the regulation of Rab GTPases and intracellular vesicular transport (PMID: 9067750, PMID: 16936131). REP-1 is involved in trafficking of Rab proteins in the cell. And REP-1 binds newly synthesized Rab proteins and presents them to the catalytic site of RabGGTase (PMID: 19427510). RAB-27 in several neurons, such as motor neurons at neuromuscular junctions, requires REP-1 for normal synaptic transmission responsible for aldicarb sensitivity (PMID: 19090809). REP-1 acts as a component of the protein complex. There are two similar Rab escort proteins (75% sequence identity): REP-1 and REP-2. A 95-kDa protein (previously designated component A and now called REP-1) is present (PMID: 8294464, PMID: 17140818).

Protocols

Product Specific Protocols
WB protocol for CHM antibody 26251-1-APDownload protocol
IHC protocol for CHM antibody 26251-1-APDownload protocol
IF protocol for CHM antibody 26251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols