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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CHMP1A Polyclonal antibody

CHMP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15761-1-AP

Synonyms

KIAA0047, hVps46-1, hVps46 1, Chromatin-modifying protein 1a, chromatin modifying protein 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CHMP1A Polyclonal antibody (15761-1-AP)

    WB analysis of HEK-293 using 15761-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CHMP1A Polyclonal antibody (15761-1-AP)

    WB analysis of A431 using 15761-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CHMP1A Polyclonal antibody (15761-1-AP)

    WB analysis of HeLa using 15761-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    WB analysis of mouse lung using 15761-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    WB analysis of mouse kidney using 15761-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CHMP1A Polyclonal antibody (15761-1-AP)

    IP experiment of HEK-293 using 15761-1-AP

    IP result of anti-CHMP1A (IP:15761-1-AP, 3ug; Detection:15761-1-AP 1:500) with HEK-293 cells lysate 880ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of mouse brain using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of mouse brain using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of human skin using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of human skin using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of mouse testis using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of mouse testis using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of human lung using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHMP1A Polyclonal antibody (15761-1-AP)

    IHC staining of human lung using 15761-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHMP1A Polyclonal antibody (15761-1-AP)

    IF Staining of HepG2 using 15761-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15761-1-AP (CHMP1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15761-1-AP targets CHMP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP1A fusion protein Ag8389 相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 1A
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 33 kDa, 25 kDa
GenBank accession numberBC007527
Gene symbol CHMP1A
Gene ID (NCBI) 5119
RRIDAB_2229399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).

Protocols

Product Specific Protocols
WB protocol for CHMP1A antibody 15761-1-APDownload protocol
IHC protocol for CHMP1A antibody 15761-1-APDownload protocol
IF protocol for CHMP1A antibody 15761-1-APDownload protocol
IP protocol for CHMP1A antibody 15761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
View Article
humanWB

J Extracell Vesicles

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation

Authors - Pauline P Marie
View Article
mouseWB,IF

Nat Commun

A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.

Authors - Yuting Guan
  • KO Validated
View Article
mouseWB

Nat Commun

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Authors - Luana L Scheffer
View Article
human,mouseIHC

Cell Rep

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Authors - Michael E Coulter
  • KO Validated
View Article
humanWB

Cell Rep

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Authors - Inna Goliand
  • KD Validated
View Article