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KD/KO Validated

CHMP1A Polyclonal antibody

Name: CHMP1A Polyclonal antibody
Brand: Proteintech
SKU: 15761-1-AP

CHMP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15761-1-AP

Synonyms

KIAA0047, hVps46-1, hVps46 1, Chromatin-modifying protein 1a, chromatin modifying protein 1A

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15761-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15761-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15761-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15761-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15761-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15761-1-AP
IP result of anti-CHMP1A (IP:15761-1-AP, 3ug; Detection:15761-1-AP 1:500) with HEK-293 cells lysate 880ug.
IHC staining of mouse brain using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 15761-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15761-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15761-1-AP (CHMP1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15761-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

15761-1-AP targets CHMP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHMP1A fusion protein Ag8389 相同性解析による交差性が予測される生物種
Full Name	chromatin modifying protein 1A
Calculated molecular weight	196 aa, 22 kDa
Observed molecular weight	33 kDa, 25 kDa
GenBank accession number	BC007527
Gene symbol	CHMP1A
Gene ID (NCBI)	5119
RRID	AB_2229399
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).

Protocols

Product Specific Protocols
WB protocol for CHMP1A antibody 15761-1-AP	Download protocol
IHC protocol for CHMP1A antibody 15761-1-AP	Download protocol
IF protocol for CHMP1A antibody 15761-1-AP	Download protocol
IP protocol for CHMP1A antibody 15761-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
human	WB	J Extracell Vesicles Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation Authors - Pauline P Marie View Article
mouse	WB,IF	Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis. Authors - Yuting Guan KO Validated View Article
mouse	WB	Nat Commun Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. Authors - Luana L Scheffer View Article
human,mouse	IHC	Cell Rep The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Authors - Michael E Coulter KO Validated View Article
human	WB	Cell Rep Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM. Authors - Inna Goliand KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHMP1A Polyclonal antibody

CHMP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 15761-1-AP

WB analysis of mouse kidney using 15761-1-AP

WB analysis of A431 using 15761-1-AP

WB analysis of HeLa using 15761-1-AP

WB analysis of mouse lung using 15761-1-AP

IP experiment of HEK-293 using 15761-1-AP

IHC staining of mouse brain using 15761-1-AP

IHC staining of mouse brain using 15761-1-AP

IHC staining of mouse testis using 15761-1-AP

IHC staining of mouse testis using 15761-1-AP

IHC staining of human skin using 15761-1-AP

IHC staining of human skin using 15761-1-AP

IHC staining of human lung using 15761-1-AP

IHC staining of human lung using 15761-1-AP

IF Staining of HepG2 using 15761-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation

A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

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