Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 17 publications below |
| WB | See 29 publications below |
| IF | See 24 publications below |
| IP | See 1 publications below |
Product Information
10477-1-AP targets CHMP2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHMP2A fusion protein Ag0719 相同性解析による交差性が予測される生物種 |
| Full Name | chromatin modifying protein 2A |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC002502 |
| Gene Symbol | CHMP2A |
| Gene ID (NCBI) | 27243 |
| RRID | AB_2079470 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP2A, also known as Vps2-1, belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in the degradation of surface receptor proteins and the formation of endocytic multivesicular bodies (MVBs). It has been shown that CHMP2A can interact with and regulate the function of the AAA-ATPase VPS4B (PMID: 15173323). CHMP2A is also reported to be involved in HIV budding (PMID: 21396898; 23051622).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP2A antibody 10477-1-AP | Download protocol |
| IHC protocol for CHMP2A antibody 10477-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
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Nature Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
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Nat Cell Biol Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.
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Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. |