Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 12527-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 12527-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12527-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12527-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12527-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12527-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12527-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 12527-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 12527-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of PFA fixed cells using 12527-1-AP
IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman.
IF Staining of HepG2 using 12527-1-AP
Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

CHMP2B Polyclonal antibody

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12527-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI-84, CHMP2.5, chromatin modifying protein 2B, Chromatin-modifying protein 2b, DMT1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, human heart tissue, mouse brain tissue, human ileum tissue, human kidney tissue, human placenta tissue, human brain tissue
Positive IHC detected in	mouse brain tissue, human brain tissue, human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, PFA fixed cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 7 publications below
IP	See 2 publications below

Product Information

12527-1-AP targets CHMP2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3222 Product name: Recombinant human CHMP2B protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-213 aa of BC001553 Sequence: MASLFKKKTVDDVIKEQNRELRGTQRAIIRDRAALEKQEKQLELEIKKMAKIGNKEACKVLAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQKTLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAKAPSAARSLPSASTSKATISDEEIERQLKALGVD 相同性解析による交差性が予測される生物種
Full Name	chromatin modifying protein 2B
Calculated molecular weight	24 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC001553
Gene Symbol	CHMP2B
Gene ID (NCBI)	25978
RRID	AB_10603358
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UQN3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

Protocols

Product Specific Protocols
IF protocol for CHMP2B antibody 12527-1-AP	Download protocol
IHC protocol for CHMP2B antibody 12527-1-AP	Download protocol
WB protocol for CHMP2B antibody 12527-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
pig	WB,IP,IF	PLoS Pathog Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection. Authors - Chun-Chun Liu View Article
human	IF	Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation. Authors - Kun Gao View Article
mouse	WB	J Neurosci Nascent Aβ42 Fibrillization in Synaptic Endosomes Precedes Plaque Formation in a Mouse Model of Alzheimer's-like β-Amyloidosis Authors - Elizabeth A Eckman View Article
human	WB	Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. Authors - Yi Huo View Article
mouse	IF	Sci Rep VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis. Authors - Carolyn Ott View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of NIH/3T3 using 12527-1-AP

WB analysis of human ileum using 12527-1-AP

WB analysis of human kidney using 12527-1-AP

WB analysis of human heart using 12527-1-AP

WB analysis of human placenta using 12527-1-AP

WB analysis of human brain using 12527-1-AP

WB analysis of mouse brain using 12527-1-AP

IHC staining of mouse brain using 12527-1-AP

IHC staining of mouse brain using 12527-1-AP

IHC staining of human liver cancer using 12527-1-AP

IHC staining of human liver cancer using 12527-1-AP

IHC staining of human brain using 12527-1-AP

IHC staining of human brain using 12527-1-AP

IHC staining of human brain using 12527-1-AP

IHC staining of human liver using 12527-1-AP

IHC staining of human liver using 12527-1-AP

IHC staining of human brain using 12527-1-AP

IF Staining of HepG2 using 12527-1-AP

IF Staining of PFA fixed cells using 12527-1-AP

IF Staining of HepG2 using 12527-1-AP

CHMP2B Polyclonal antibody

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Nascent Aβ42 Fibrillization in Synaptic Endosomes Precedes Plaque Formation in a Mouse Model of Alzheimer's-like β-Amyloidosis

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.