Validation Data Gallery
Tested Applications
| Positive WB detected in | TT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
16256-1-AP targets CHMP4C in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHMP4C fusion protein Ag9279 相同性解析による交差性が予測される生物種 |
| Full Name | chromatin modifying protein 4C |
| Calculated molecular weight | 233 aa, 26 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC014321 |
| Gene Symbol | CHMP4C |
| Gene ID (NCBI) | 92421 |
| RRID | AB_3669233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP4C (Charged multivesicular body protein 4c), also known as SHAX3. It is expected to be located in the cytoplasm and membrane, which is expressed in heart, spleen and kidney. CHMP4C is one of the charged multivesicular protein (CHMP), and is involved in the composition of the endosomal sorting complex required for transport III (ESCRT-III), facilitating the necessary separation of daughter cells. CHMP4C has been proposed to be involved in the progression of different carcinomas. The molecular weight of CHMP4C is 26 kDa. Phosphorylated at Ser-210 by AURKB during cytokinesis: together with ZFYVE19/ANCHR, phosphorylated CHMP4C retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP4C antibody 16256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Heliyon Analysis of cell death-related genes to evaluate the prognostic and immunotherapeutic value in bladder cancer |