Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse testis using 16278-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16278-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 16278-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16278-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16278-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 16278-1-AP
IP result of anti-CHMP6 (IP:16278-1-AP, 4ug; Detection:16278-1-AP 1:500) with mouse testis tissue lysate 2400ug.
IHC staining of human colon cancer using 16278-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 16278-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CHMP6 Polyclonal antibody

CHMP6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16278-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Vps20, Vacuolar protein sorting-associated protein 20, hVps20, Chromatin-modifying protein 6, chromatin modifying protein 6

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human stomach tissue, human colon cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

16278-1-AP targets CHMP6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHMP6 fusion protein Ag9357 相同性解析による交差性が予測される生物種
Full Name	chromatin modifying protein 6
Calculated molecular weight	201 aa, 23 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC010108
Gene Symbol	CHMP6
Gene ID (NCBI)	79643
RRID	AB_2079498
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96FZ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP6 antibody 16278-1-AP	Download protocol
IHC protocol for CHMP6 antibody 16278-1-AP	Download protocol
IP protocol for CHMP6 antibody 16278-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
canine	WB	Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. Authors - Eva P Karasmanis View Article
human	WB	J Virol A non-envelopment role for the ESCRT-III complex during HCMV infection. Authors - Nicholas T Streck View Article
human	WB	J Virol Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread. Authors - Nicholas T Streck View Article
human	WB,IF	Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy Authors - Takashi Nozawa KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 16278-1-AP

WB analysis of Jurkat using 16278-1-AP

WB analysis of U-937 using 16278-1-AP

WB analysis of A431 using 16278-1-AP

WB analysis of A375 using 16278-1-AP

IP experiment of mouse testis using 16278-1-AP

IHC staining of human colon cancer using 16278-1-AP

IHC staining of human stomach using 16278-1-AP

CHMP6 Polyclonal antibody

CHMP6 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

A non-envelopment role for the ESCRT-III complex during HCMV infection.

Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy