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KD/KO Validated

CHMP6 Polyclonal antibody

Name: CHMP6 Polyclonal antibody
Brand: Proteintech
SKU: 16278-1-AP

CHMP6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16278-1-AP

Synonyms

Vps20, Vacuolar protein sorting-associated protein 20, hVps20, Chromatin-modifying protein 6, chromatin modifying protein 6

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse testis using 16278-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16278-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 16278-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16278-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16278-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 16278-1-AP
IP result of anti-CHMP6 (IP:16278-1-AP, 4ug; Detection:16278-1-AP 1:500) with mouse testis tissue lysate 2400ug.
IHC staining of human colon cancer using 16278-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 16278-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16278-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human stomach tissue, human colon cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

16278-1-AP targets CHMP6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHMP6 fusion protein Ag9357 相同性解析による交差性が予測される生物種
Full Name	chromatin modifying protein 6
Calculated molecular weight	201 aa, 23 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC010108
Gene symbol	CHMP6
Gene ID (NCBI)	79643
RRID	AB_2079498
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP6 antibody 16278-1-AP	Download protocol
IHC protocol for CHMP6 antibody 16278-1-AP	Download protocol
IP protocol for CHMP6 antibody 16278-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
canine	WB	Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. Authors - Eva P Karasmanis View Article
human	WB	J Virol A non-envelopment role for the ESCRT-III complex during HCMV infection. Authors - Nicholas T Streck View Article
human	WB	J Virol Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread. Authors - Nicholas T Streck View Article
human	WB,IF	Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy Authors - Takashi Nozawa KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHMP6 Polyclonal antibody

CHMP6 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of mouse testis using 16278-1-AP

WB analysis of Jurkat using 16278-1-AP

WB analysis of U-937 using 16278-1-AP

WB analysis of A431 using 16278-1-AP

WB analysis of A375 using 16278-1-AP

IP experiment of mouse testis using 16278-1-AP

IHC staining of human colon cancer using 16278-1-AP

IHC staining of human stomach using 16278-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

A non-envelopment role for the ESCRT-III complex during HCMV infection.

Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy