Validation Data Gallery
Tested Applications
| Positive WB detected in | Tunicamycin treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
60304-1-Ig targets CHOP; GADD153 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CHOP; GADD153 fusion protein Ag7354 相同性解析による交差性が予測される生物種 |
| Full Name | DNA-damage-inducible transcript 3 |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC003637 |
| Gene Symbol | CHOP |
| Gene ID (NCBI) | 1649 |
| RRID | AB_2881418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35638 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHOP; GADD153 antibody 60304-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. | ||
Cell Death Dis YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma. | ||
Hum Mol Genet The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa. | ||
Oncotarget Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells. | ||
Apoptosis Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation. | ||
J Virol ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress |